Dilution of edta with a final concentration

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  1. The molecular weight of anhydrous calcium chloride is 110.99. How many grams do you need for 100 ml of 50 mM calcium chloride?
  2. Hydrogen peroxide is supplied in a 30% solution. If your protocol calls for using a 0.1% solution, how would you make 20mL?
  3. You are given a stock solution of EDTA at a concentration of 5mM, how would you dilution of EDTA with a final concentration of 5mM in a final volume of 250mL?
  4. You need to prepare a stock solution of medium for your culture cells, which usually includes liquid salt solution and bovine serum. Your liquid salt solution is supplied in a 10X concentration, and you need to dilute it to 1X for use. You also need to add 100% fetal bovine serum for a final concentration of 10%. How would you make up 5 liters of this culture media using water as your solvent?

Reference no: EM1394852

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