Digested the fragment with both enzymes together

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Reference no: EM13527016

You’ve cloned a 2-kb fragment of DNA from the G38APO8 gene into a bacterial cloning vector and want to construct a restriction map of the insert. You amplify the 2-kb insert using PCR, purify it, and subject it to differential digestion with the enzymes EcoRI and HindIII, gel-fractionate the digests, and visualize the restriction patterns by staining the gels with E-Z vision to generate the following results. Using this map, draw and label what you would expect the gel banding pattern to be for each of the two restriction enzymes separately and if you digested the fragment with both enzymes together (draw three gels). Numbers on the map indicate the number of base pairs between cut sites

since the pic isnt working
EcoRI HindIII EcoRI HindIII EcoRi
_150________600______100_____525_______________425____200

The first EcoRI is close to 150, the second is closer to 100 than 525 and the last is in the middle of 425 and 200
HindIII is closest to 100 than 600 and its in the middle of 525 and 425 but slightly closer to 425

Reference no: EM13527016

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