Differentiate myeloid progenitors into microglia

Reference no: EM133610739

Question

You are trying to differentiate myeloid progenitors into microglia by over-expressing the transcription factor PU.1. You first test if your overexpression method worked. What experimental approaches would you use to test whether your construct results in mRNA and protein expression in these cells? Please describe the approaches, including technical and experimental controls, and the expected results.

Reference no: EM133610739

Questions Cloud

Describe the macrophage feed-forward loop : Arrange the items in order to describe the macrophage feed-forward loop present in the intestines when a pathogen breaches the epithelial barrier.

Slight paralysis of one limb is called monoparesis : Slight paralysis of one limb is called monoparesis. The term coma means fainting. A manner of walking is called ataxia.

Arabian imported light breed stand out amongst other breeds : Why does the Arabian imported light breed stand out amongst other breeds. This might be physical, behavioral or regional characteristics.

Blood vessels-lymphatic vessels and fatty tissue : The lesion exceed the epidermis layer and entered into dermis which is richly supplied with nerve fibers, blood vessels, lymphatic vessels and fatty tissue.

Differentiate myeloid progenitors into microglia : You are trying to differentiate myeloid progenitors into microglia by over-expressing the transcription factor PU.1.

Health conditions that restrict or narrow blood vessels : An umbrella term that includes health conditions that restrict or narrow blood vessels, which can lead to a heart attack

Description of each phenotype combination present : Identify and give a brief description of each phenotype combination present -remember this is a dihybrid cross, there should be four characteristic combinations

Inherited neurodegenerative disease : Tay-Sachs disease is an inherited neurodegenerative disease and lipid metabolism disorder.

Structure and function of arteries-veins and capillaries : Compare and contrast the structure and function of arteries, veins, and capillaries. Trace blood through the systemic, pulmonary, and hepatic portal circulation

User Account

All Pages