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Differences between endospore and vegetative cell

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Exercise 5 Post-Lab Questions and Reflections

Part A: The Endospore Stain

1. How are the differences between the endospore and the vegetative cell highlighted in the endospore staining procedure?

2. Why must steam be used to force malachite green into the endospore?

3. What disease would you suspect if you found endospore-forming bacteria in exudate from a patient's wound or feces?

4. What other diseases can be diagnosed with the endospore stain?

5. Consider the following information: The red-stained bacilli are the vegetative forms of Bacillus megaterium, and the green oval-shaped structures are endospores. Given this information, reflect on the results of your endospore stains. Did your results reflect the structures and stains described above? Why or why not?

Reference no: EM132655128

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