Difference in length of a dna molecule

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QuestionCells are capable of replicating their own DNA, which amounts to billions of base pairs, quite efficiently, and yet in a PCR or sequencing reaction we are only able to accurately replicate <2000 base pairs. This is approximately the maximum length, regardless of the primers used. What difference(s) may account for the length of a DNA molecule produced in a cell vs. in a tube? List three differences that may apply AND list how teach of them might affect fragment/molecule length.

Reference no: EM1385547

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