Reference no: EM133541060

1. Aerobic/Anaerobic Growth

1. What was the purpose of this lab?

2. Understand the difference between aerobic, anaerobic, facultative organisms. (Describe the oxygen preference of each m/o used in this lab, using these terms)

3. Be able to identify the type of organism growing in a shake/agar deep tube.

4. Why were shake tubes used for this exercise and not agar slants or broth?

2.  Identification of Enteric Gram Negative rods

1. What color is a (+) phenol red fermentation test? What does a (+) test tell you about the organism?

2. What is the indicator in the phenol red fermentation test? What does it indicate?

3. What does the durham tube tell you?

4. Understand the significance between the MR and VP tests.

5. Be able to read a positive and negative test for each of the biochemical tests used in this lab.

6. Know whether or not you need to add a reagent to the test. Are there any pH indicators?

7. Understand what each test is telling you about the organism.

8. Know whether the agar plates used in this lab are selective, differential, or both. What does growth on the plates indicate? (review the MacConkey, HE, and XLD plates)

3. Skin lab

1. What types of organisms were we looking for in this exercise?

2. Why did we incubate some plates in the anaerobic chamber?

3. What types of organisms could grow on both the aerobic and anaerobic plate? Which organisms on the skin grew only on the aerobic plate? What is the gram reaction of each?

4. Why did we use a shake/melted agar deep tube?

5. What does a (+) glucose brom cresol purple slant look like? What does a positive test tell you about the organism?

6. What types of organisms grow on Mannitol Salt Agar? What can you determine about an organism that grows on a MSA plate?

7. How did you determine the species of Staphylococcus?

8. Describe the coagulase test: what does it indicate? What does a positive result look like?

9. Know the coagulase results for Staphylococcus aureus and Staphylococcus epidermidis

10. Describe Propionibacterium: colony characteristics on TSY plate. Aerobe or anaerobe? What does it look like under the microscope?

4. Streptococci and Respiratory Microbes

1. What types of organisms are normal biota of the throat? What type of agar was used to grow the throat flora?

2. Be able to identify the different types of hemolysis on a blood agar plate.

3. What type(s) of hemolysis are considered normal flora? potential pathogens?

4. What does a (+) bile esculin and (+) salt broth test look like? What kind of organism can grow on the bile esculin slant and the salt broth?

5. What is the catalase test?

6. How do you distinguish between the Staphylococcus and Streptococcus genera? (What test differentiates them?)

7. What type of hemolysis will Streptococcus pyogenes present as?

8. What organism causes "strep throat"?

5. Antibiotics

1. What are antibiotics?

2. What are the clear zones around the antibiotic discs called?

3. What does the Kirby-Bauer test tell you about an organism?

4. How do you determine antibiotic resistance or sensitivity of the organism?