Determining the theoretical yield of rna

Reference no: EM1391783

Question: Compute the concentration of your undiluted RNA sample using Beer's law.

extinction coefficient = 25 ul/ug/cm
l = 1 cm
A260 concentration = 3.4 micrograms/mL
A260/A280 ratio concentration = 1.98 micrograms/mL

Question: Suppose that the cells grow at about 10^5 cells per square cm and contain about 10pg of RNA per cell, what is the theoretical yield of RNA?

Reference no: EM1391783

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