Determining factor for melting temperature of nucleic acid

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1. Draw a standard A-T base pair.

2. Draw a standard G-C base pair

3. Define "melting temperature" for a nucleic acid. Define "annealing" for a nucleic acid. What is the most major determining factor for melting temperature of a nucleic acid?

4. Explain why deoxyribose takes the C2' endo conformation in B-form DNA, and why ribose takes the C3' endo conformation in RNA.

5. Another difference between DNA and RNA is that RNA can undergo base catalyzed cleavage of its own phosphodiester backbone. Draw the mechanism for this cleavage and explain why this does not occur in DNA.

6. You are speaking to a friend who has some biology and chemistry background but has not taken biochem yet. Write a succinct description of a riboswitch for them.

7. Some Adenosine RNA nucleotides are covalently modified by methylation at the N6 position. Draw N6-methyladenosine. Would N6-methyladenosine be able to participate in a standard, Watson-Crick base pair?

Reference no: EM133261397

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