Determine which ion exchanger and optimum ph of the buffer

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A protein of interest has an exceptionally acidic pI of around pH 5.5 whereas the rest of the other proteins have an overall basic pI of around 7.5. Which ion-exchanger (cation or anion) and optimum pH of the buffer would you recommend so that the protein of interest would elute from the column without binding?

Reference no: EM1395292

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