Determine values for the dissociation constant

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Reference no: EM13153521

Vitamin D was added to reaction vessels at different concentrations containing its receptor protein (at a fixed concentration). After equilibrium was reached, the amount of Vitamin D bound to the protein was determined. Here are the results determined

Vit.D nmol/liter RL concentration
nmol/liter
0.2 0.9
0.3 1.7
0.7 3.1
1.4 4.9
2.8 7.0
5.5 9.0
11.0 10.4

Draw a graph to demonstrate the amount of vitamin D bound as a function of its concentration. Use the double reciprocal method to determine values for the dissociation constant and the total amount of receptor present?

Reference no: EM13153521

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