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Vitamin D was added to reaction vessels at different concentrations containing its receptor protein (at a fixed concentration). After equilibrium was reached, the amount of Vitamin D bound to the protein was determined. Here are the results determined
Vit.D nmol/liter RL concentrationnmol/liter0.2 0.90.3 1.70.7 3.11.4 4.92.8 7.05.5 9.011.0 10.4
Draw a graph to demonstrate the amount of vitamin D bound as a function of its concentration. Use the double reciprocal method to determine values for the dissociation constant and the total amount of receptor present?
You just harvested either a BM or PB sample and found very few metaphases under the phase-contrast microscope. What might be the possible reason in terms of specimen handling, culture setup, harvesting, and slide preparation?
Discuss two essential characteristics of a hereditary material. Briefly explain how these characteristics are demonstrated by DNA.
To make up a solution of phosphate buffered saline, you need 5mM Na2HPO4 (anhydrous) (FW: 141.96), 0.15 M NaCl (FW: 58.44), and 1 mM KH2PO4
Explain why the increased prevalence of sickle cell disease among African Americans actually has more to do with the environment than skin color or other phenotypes used to define races.
Explain how each of these ideas works and find ways to link between them. Present examples from your experience to demonstrate an understanding of the principles involved.
Some human pathogenic bacteria are resistant to most antibiotics. How would you prove a bacterium is resistant to antibiotics using laboratory culture techniques?
Do you think that using recombinant DNA technologies to change the genetic composition of human egg cell is ever justified? If so, what restrictions have to be placed on such a use.
As we know that mean arterial blood pressure = CO cardiac output x total peripheral resistance (TPR), what might be a normal mechanism to compensate for elevated blood pressure?
The following sequence represents the region upstream (5') of a coding region where the first codon is indicated. Using the sequence provided for the 3' end of the E. coli 16S rRNA,
you are instructed to add 1.0ml out of 5.0ml of an undiluted sample to 99ml of sterile diluent. instead you add all of 5.0ml to the 99ml. what was the intended dilution and what was the actual dilution?
In a co-evolved predator-prey relationship, does predation rate operate under directional selective pressure or stabilizing selective pressure?
Goldfish skin and chicken egg yolks typically have a yellow, orange, or reddish-orange color. However, these tissues are practically colorless if the respective animal is not permitted to eat photosynthetic plant tissue. Propose a hypothesis to ex..
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