Reference no: EM1384349

The information to the below were collected for the bovine carbonic anhydrase-catalyzed reaction described by

CO2 (aq) + H2O (l) -->H+ (aq) + HCO3- (aq)

[CO2] (mmol * L^-1)	R (�µmol * L^-1 * s^-1)
1.25	28.74
2.50	48.61
5.00	80.28
20.00	155.59

Use these values to determine the value of KM, the Michaelis-Menten constant. Answer the four parts below.

a) If you were to plot this data to graphically determine Rmax and KM using a Lineweaver-Burk plot, what would you plot for the following data? If you would plot the original data, enter the original value in the corresponding blank.

[CO2] (mmol * L^-1) graph as R (�µmol * L^-1 * s^-1) graph as

2.50 --> ________ 48.61 --> ________

5.00 --> ________ 80.28 --> ________

b) Calculate the slope using the data points from part (a). Then determine the y-intercept using the second point.

Slope= _______ s y-intercept= ___________ L * s * mmol^-1

c) Use the y-intercept to calculate Rmax.

Rmax=___________ mmol * L^-1 * s^-1

d) Use Rmax and the slope to calculate Km.

Km=___________ mmol * L^-1