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A patient was identified with severely depleted levels of a secreted growth factor (GF1), normally found in the circulation. Secretion of all other proteins was not affected, suggesting that there is a defect in the GF1 protein. Cells have been isolated from the patient (secretory defect 1 - SD1 cells) and can be grown in culture in the lab. Cells from an unaffected individual are also available (normal control - NC cells). Preliminary experiments using Western blotting have shown that both NC cells and SD1 cells are synthesising GF1 but, in the case of SD1 cells, it is not being secreted into the culture medium.
To investigate the secretory defect, you decide to use immunofluorescence microscopy to determine the subcellular localisation of GF1 within the SD1 and NC cells.
From your results you discover that, in the SD1 cells, the GF1 protein is predominantly in the cytosol and not associated with any particular organelle. With the aid of a diagram, describe how proteins like this growth factor are normally targeted to the ER lumen.
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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