Determine the stoichiometric relationship

Assignment Help Biology
Reference no: EM135823

Q. Signalling molecules can regulate transcription and as a result control cell differentiation during development. One signaling molecule, sonic hedgehog (shh), has been shown to regulate the dorsoventral patterning of the neural tube.

a) In what tissue is shh produced and what is the evidence that this tissue regulates the ventral pattern of the neural tube (even before this tissue was known to produce shh)?

b) Illustrate one experiment that shows that shh is the critical molecule produced by the inducing tissue referred to in (a).

c) How is the gradient of shh converted into differential transcriptional activation? What I am looking for here is how the investigators determined the stoichiometric relationship between shh gradient and active Gli transcription factors. Describe the experiment that elucidated this relationship. I do not require to have all the details but I want you to describe the experimental approach that was taken, the expression constructs that were generated, how these constructs were delivered to the neural tube and what the results were.

Reference no: EM135823

Questions Cloud

How much money will you require to deposit to get goals : Suppose a 10% interest rate, how much money will you require to deposit each year to be able to meet these financial goals?
Oil and gasoline prices in the united states : Oil and gasoline prices are a concern in the United States. Why does this economic problem exist from a supply and demand perspective, what can be done to improve resource allocations.
What are the genotypes of the parents : What information does it not provide that may be useful for deciding on conservation efforts. What are the genotypes of the parents.
Evaluate tamras actual factory overhead costs : Evaluate Tamra's actual factory overhead costs for February 2013. Direct labor costs and Actual per-unit direct material for February 2013 were $24.30 and $10.95. Determine actual total product cost for February.
Determine the stoichiometric relationship : Illustrate one experiment that shows that shh is the critical molecule produced by the inducing tissue referred to in (a). what is the evidence that this tissue regulates the ventral pattern of the neural tube (even before this tissue was known to pr..
Powerfull strategy to make people aware : Advertising is powerfull strategy to make people aware about company products and services and for this case is to emphasize reliability and low price, this effort will help the company to sustain in this area and to develop a customer franchise a..
Evaluate effect on classification and carrying value : Evaluate the effect on classification, carrying value, and earnings for each of the given situations. Discuss whether U.S. GAAP under SFAS No. 115 or the needs of IAS.
Fractional reserve banking system : The banking system is a fractional reserve banking system with a desired reserve deposit ratio. Banking system is a fractional reserve banking system.
Levels of eco-consciousness and social-responsibility : Do you deliberate its levels of eco-consciousness and social-responsibility set a realistic model of environmental performance for other manufacturing companies.

Reviews

Write a Review

Biology Questions & Answers

  Immune response to infectious disease

It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.

  A review on advanced glycated end products (ages)

This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.

  Plastic as a soil stabilizer

Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.

  Principles of microbiology

This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.

  List the biologic functions

Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?

  Biologic function relationships

Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.

  Case study on patient in the haematology laboratory

Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.

  Use of pcr and genetic approaches in biotechnology

The use of PCR and genetic approaches in biotechnology

  Describe the role of this enzyme in honey

Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.

  Genetic problems

What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?

  Prepare an essay on nosocomial infection

Prepare an essay on nosocomial infection.

  Monitoring and recording the blood pressure

To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.

Free Assignment Quote

Assured A++ Grade

Get guaranteed satisfaction & time on delivery in every assignment order you paid with us! We ensure premium quality solution document along with free turntin report!

All rights reserved! Copyrights ©2019-2020 ExpertsMind IT Educational Pvt Ltd