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Q. Signalling molecules can regulate transcription and as a result control cell differentiation during development. One signaling molecule, sonic hedgehog (shh), has been shown to regulate the dorsoventral patterning of the neural tube.
a) In what tissue is shh produced and what is the evidence that this tissue regulates the ventral pattern of the neural tube (even before this tissue was known to produce shh)?
b) Illustrate one experiment that shows that shh is the critical molecule produced by the inducing tissue referred to in (a).
c) How is the gradient of shh converted into differential transcriptional activation? What I am looking for here is how the investigators determined the stoichiometric relationship between shh gradient and active Gli transcription factors. Describe the experiment that elucidated this relationship. I do not require to have all the details but I want you to describe the experimental approach that was taken, the expression constructs that were generated, how these constructs were delivered to the neural tube and what the results were.
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Though the bone was properly set, by the time boy was 16 it was apparent that the injured lower limb was shorter than normal one. Elucidate why this difference occurred. What caused her symptoms? Was her condition because of an infection or intoxicat..
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The Verhulst's logistic model has been practical to the natural growth of the halibut population in certain areas of the Pacific Ocean. Let NN, measured in kg, be the total mass (or biomass) of the halibut population at time tt.
An inversion heterozygote possesses a normal chromosome and one with an inversion. The gene orders in these 2 chromosomes are shown below- Normal 1 2 3 4 5 6 7 8 9 10 o.
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