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You have a 50 µl of pEGFP template DNA from which you want to amplify the GFP ORF. Previously an aliquot of this DNA was diluted 1/100 in H2O and the absorbance at 260nm was measured using a nanodrop spectrophotometer. The absorbance (minus blank) was 0.34. (Note - an absorbance of 1 at 260nm corresponds to a concentration of 50 μg/ml for double-stranded DNA).
You need to add 25 ng of pEGFP template DNA in total to your reaction. Determine the stock concentration of your template DNA and come up with a protocol for diluting it to an appropriate concentration to allow you to add 25 ng to your PCR reaction - the volume of pEGFP should be no greater than 5 µl.
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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