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Question: A linear fragment of DNA that is 22 Kb is cleaved with the individual restriction enzymes SalI, HindIII and KpnI and then in the pair wise combinations listed below. The sizes of DNA fragments seen on the resulting ethidium bromide stained gel are:SalI: 11 Kb, 6 Kb, 5 KbHindIII: 14 Kb, 8 KbKpnI: 16 Kb, 6 KbSalI and HindIII: 8 Kb, 6Kb, 5Kb, 3KbSalI and KpnI: 11 Kb, 5 Kb, 1 KbHindIII and KpnI: 8 Kb, 6 KbDetermine the restriction map for the linear DNA fragment (ie: figure out where each restriction enzymes' cut sites are located on the DNA fragment).Select the correct order in which the restriction enzyme cut sites would occur on the linear DNA fragment beginning at one end of the fragment.Select one:a. KpnI, SalI, SalI, HindIIIb. KpnI, SalI, HindIII, SalIc. HindIII, SalI, KpnI, SalId. KpnI, SalI, HindIII
Transfer of the immunologically competent T-lymphocytes from a donor into host expression different MHC antigens can have severe consequences, causing a fatal syndrome known as graft versus host disease (GVH).
define the results of the three chi-squared analyses. what do these analyses tell you about the chromosomal arrangements and the genotypes of plants 1 and 2 and the events that occured during gamete production.
A scientist randomly separated a group of 100 lab rats into two groups of 50. One group was fed regular rat chow while the other was fed the same quantity of rat chow with added ddt. Both groups were housed in the same room with the same environmenta..
Explain the mechanism of horizontal gene transfer during transduction. Describe a disease in which transduction plays a role and explain what it does
G3-15ml frozen bacterial pellet was added to the breaking buffer and a freeze-thaw procedure mixing the two solutions through sucking up the mixture up and down in the vial was performed.
Filling out two Punnett squares for a cross between a male with blood type B and a female with blood type AB. Build two Punnett squares and answer the following questions about them.
express "transamination" and "oxidative deamination"
Suppose after washing your hands, you leave ten bacterial cells on the new bar of soap. You next decide to do a plate count of the soap after it is left in the soap dish for 24 hours. You dilute 1g of the soap 1:10 and plate it on a standard plate co..
Which of these statements can apply to endocrine disruptors.
Assume you have also identified a 3rd recessive mutation that you call crimson. Wasps homozygous for crimson have a red abdomen.
Describe why dobutamine is sometimes given to patients to simulate the effect of exercise. What drug would you suggest a person take to attenuate the effect of dobutamine?
Utilize the words mesoglea, epidermis, gastrodermis, polyp, medusa, and gastrovascular cavity to describe the differences between the two forms of phylum cnidaria.
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