Reference no: EM133142272

Experiment - Optimisation of the separation of sulfanilamide and benzoic acid by capillary zone electrophoresis (CZE)

The aim of this experiment is to use factorial design to determine the optimum experimental conditions for capillary voltage, ionic strength of buffer and percentage of organic modifier, for the separation of sulphanilamide and benzoic acid using CZE. You will find model data in the proforma.

Experimental - The basic instrumental conditions are given below.

Instrument - Beckman P/ACE MDQ capillary electrophoresis system.

Software - 32 KARAT

Capillary - length = 57 cm, effective length = 50 cm, 75 μm ID.

Electrolyte - 10 mM sodium borate, 50 mM boric acid, pH 8.6.

Injection - pressure, 0.5 psi, 10 seconds.

Separation - Voltage, 20 kV and 25 kV.

Polarity - Normal.

Detection - UV 215 nm.

Part 1 - Effect of voltage

Students would have prepared two solutions in the 10 mL volumetric flasks provided: 200 ppm benzoic acid and 100 ppm sulphanilamide. Run your samples in duplicate. For two level analysis high and low values for each variable must be chosen. You will not have time to carry out the eight experiments required when three factors are involved and therefore you can only investigate a single factor (Le. voltage). Two experiments will be carried out; one at the high level and one at the low level (see model data Table 1).

Part 2 - Analysis of factorial design (see model data Table 2).

Report - Complete and upload your report/ presentation/ poster/ journal article as instructed on the proforma, which will also include your model data for analysis.

Attachment:- Experiment Assignment Files.rar