Determine the km of mutant proteases

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Reference no: EM1394874

You have successfully developed two new inhibitors (Alice-1 & Alice-2) for the mutant peptidase mentioned in question To understand how the inhibitors work, you carried out enzyme assays measuring the ability of the mutant peptidase to cleave the substrate in the absence and presence of the two inhibitors and obtained following data:

Using the entire data set to make a diagnostic plot, answer the following:

	V0, ?M/sec
[S], ?M	No inhibitor	+ Alice-1 (10 nM)	+ Alice-2 (10 nM)
2	0.062	0.006	0.042
4	0.118	0.012	0.080
8	0.210	0.024	0.157
16	0.348	0.045	0.256

(a) Does Alice-1 or Alice-2 greatly affect the ability of the mutant peptidase to carry out the cleavage reaction (kcat)?

(b) Determine the Km of the mutant proteases for its substrate in the absence and presence of inhibitors.

Reference no: EM1394874

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