Determine the km and vmax values for the enzyme

Reference no: EM1397853

The following kinetic data were obtained for an enzyme in the absence and in the presence of an inhibitor at a 5 mM concentration. You can assume that the same amount of enzyme was present in each assay, [ET]

Substrate	Rate	Rate (+inhibitor)
mM	mM/min	mM/Min
1	1.3	0.8
2	2	1.2
3	2.8	1.7
8	3.6	2.2
12	4	2.4

Determine the Km and Vmax values for the enzyme in the presence and presence of the inhibitor. (Show your graphical results!) Report Km or Vmax values in mM or mM/min as appropriate, and indicate which type of inhibition is demonstrated by the inhibitor? You MUST explain your reasoning

Reference no: EM1397853

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