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1) Describe principle behind various whole genome sequencing technologies. Describe application of one whole genome sequencing from literature for providing deeper insight into biological function.
2) You have isolated a novel transcription factor. How will you determine the genes regulated by transcription factor in the whole genome
3) How will you determine the transcription factor binding sites in the whole genome?
4) You have performed yeast two hybrid assay to identify proteins that interact with superman and identified a gene coding for kryptonite that interacts with superman. What experiments you will perform to demonstrate that the superman interacts with kryptonite in-vivo (inside nucleus in the cell)?
Give atleast two examples.
5) What is microRNA. Provide one example from literature how microRNA regulates mRNA.
Compare the control of gene regulation in eukaryotes and prokaryotes at the level of initiation of transcription.
List whether the movement is diffusion, osmosis, active transport, or bulk flow. Clarify the adaptations that increase the effectiveness of this movement. what is the meaning of this observation.
Suppose that one hundred percent efficiency of energy conservation, how many moles of ATP can be synthesized under standard conditions by the complete oxidation of 1 mol of palmitate?
Define imperfect adaptation and provide an anatomical or molecular example and explain why it is a case of imperfect adaption.
Create a pure-breeding double-mutant apricot, blunt line and cross it with wild-type wasps. The F 1 is wild-type in appearance.
In pea plants, allele for purple flowers (P) is dominant to the allele for white flowers (p). In a cross between the purple-flowered plant and the white-flowered plant, all 73 of the offspring plants had purple flowers. Which of the most likely descr..
Think about the fructose-1,6-bisphosphatase reaction. Calculate the free energy change if the ratio of the concentrations of the products to the concentrations of the reactants is 24.1,
The genes dumpy wings, clot eyes, and apterous wings are linked on chromosome II of Drosophila. In a series of two-point mapping crosses, the genetic distances demonstrate below were determined.
It's Thanksgiving day at the Simpsons' and Bart has removed the labels from the olive oil (primarily monounsaturated fatty acids) and canola oil (primarily polyunsaturated fatty acids) and no one can figure out which bottle is which.
Logan is a geneticist studying a blood clotting gene, Factor VIII, that contains 186, 000 base pairs that code for amino acids plus 32,400 base pairs that are non-coding. How would he classify the noncoding regions?
What happens to the average speeds of the particles in a gas when one increases that gas's temperature?
Chloroplast thylakoids can be isolated and purified for biochemical experiments. Probes to measure pH are used to measure the interior thylakoid compartment and solution surrounding the thylakoids.
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