Determine the constants vm

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The following data were obtained from enzymatic oxidation of phenol by oxidase atdifferent phenol concentrations:

S (mg/l):  10  20  30  50    60    80  90  110   130   140  150

V (mg/l.h) 5  7.5   10  12.5  13.7   15   15   12.5   9.5    7.5   5.7

a) What type of inhibition is this?

b) Determine the constants Vm, Km.

c) Determine the oxidation rate at [S] = 70 mg/l.

Reference no: EM131422734

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