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Question: A protein that tests disulfide positive is oxidized. Spectrophotometry readings are taken for both unoxidized and oxidized protein. Given the data below determine the concentration of each protein state and explain what we are observing.
Protein A(unoxidized) -> A(oxidized)
A280 2.4 ×103
A280 3.4 ×103
?280= 4.2 ×103 L mol-1 cm-1
?280=4.2 ×103 L micromol-1 cm-1
Concentration= ?
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