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20 g soil sample was added to 80 ml of water to make the final volume of 100 ml (tube A). Then, 10 ml of the ‘tube A' solution was taken and added to 90 ml of water (tube B). 1 ml of the ‘tube B' solution was taken and added to 9 ml of water (tube C). 1 ml of the ‘tube C' solution was taken and added to 99 ml of water (tube D). 10 ml of the ‘tube D' solution was taken and added to 90 ml of water (tube E). Now, 0.1 ml out of the tube C, D or E was taken and plated on agar-media plates in triplicate. The table below shows colony numbers counted, after overnight incubation.
C
D
E
TNTC (too numerous to count), 431, 378
43, 52, 33
1, 0, 6
(a) What are the fold dilutions in the tubes C, D and E against the tube A?
(b) Determine the CFU/g of soil.
(c) How many bacteria were present in 8 g of soil?
Which of the follow was necessary to this confirming experiment. Phosphorus is not present in amino acids in the protein coat of phage.
A cell with a 5 percent concentration of salt in it is placed in a solution with a 15 percent concentration of salt. Imagine that the cell is permeable to water but not to salt.
Eukaryotes, one complete set of nuclear chromosomes Note that Eukaryotes also possess a mitochondrial genome thus plants have a chloroplast genome and a mitochondrial genome What about fungi? What is the source of those organellar genomes.
An experienced goldfish breeder receives two unusual male goldfish. One is black rather than gold and other has a single fail fin rather than a split tail fin.
Modern theory suggests that the early pre-life atmosphere on Earth was a reducing one. Why is it believed that oxygen was not present as life formed on Earth.
Both endocrine and nervous system are major regulating system of the body; but nervous system has been compared to an airmail delivery system and the endocrine to pony express. Briefly explain the comparison.
how this Statement applies to replication or transcription
Show the lines of current flow to unexcited parts of the cable. Elucidate how these local currents cause the action potential to propagate. A common mistake is to confuse the currents that flow throughout the action potential the voltage-clamp curren..
Describe how the cell derives additional energy by switching . What was the key factor that determined this change.
The mutated form of hemoglobin in sickle-cell anemia results from the replacement of a glutamate residue by a valine residue at position 6 in the chain of the protein.
The endocrime and nervous system are main regulating systems of the body. However, the nervous system has been compared to an airmail delivery system, and endocrime system to the pony express. In brief explain this comparison.
Describe the three kind of the recombination among microorganisms. DNA is transferred horizontally among same generation. The phenotype of gene at end of recombination will be changed.
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