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QUESTION 1:
An Hfr strain is used to map three genes in an interrupted matingexperiment. The cross is Hfr/a+ b+ c+ rif X F-/a- b- c- rifr. (Nomap order is implied in the listing of the alleles; rifr isresistance to the antibiotic rifampicin.) The a+ gene is requiredfor the biosynthesis of nutrient A, the b+ gene for nutrient B, andthe c+ for nutrient C. The minus alleles are auxotrophs for thesenutrients. The cross is initiated at time = 0, and at varioustimes, the mating mixture is plated on three types of medium. Eachplate contains minimal medium (MM) plus rifampicin plus specificsupplements that are indicated in the following table. )The resultsfor each time interval are shown as the number of colonies growingon each plate.) Time of InterruptionSupplements Added to MM 5 min 10 min 15 min 20 minNutrients A and B 0 0 4 21Nutrients B and C 0 5 23 40Nutrients A and C 4 25 60 82Based on these data, determine the approximate location on thechromosomes of the a, b and c, genes relative to one another and tothe F factor.Choose one answer. 1. c a b F 2. a b c F 3. F a b c 4. F b a c 5. b a c F
QUESTION 2:
Two Theoretical genetic strains of a virus (a- b- c- and a+ b+c+)were used to simultaneously infect a culture of host bacteria.Of the 10,000 plaques scored, the following genotypes wereobserved. Determine the genetic map of these three genes on theviral chromosome. Decide whether interference was positive ornegative.a+ b+ c+ 4100 a- b+ c- 160a- b- c- 3990 a+ b- c+ 140a+ b- c- 740 a- b- c+ 90a- b+ c+ 670 a+ b+ c- 110Choose one answer. 1. a b c, positive 2. b a c, negative 3. c a b, positive 4. a c b, negative 5. a c b, positive
what is acute and convalescent sera? If the titer of the convalescent serum is much grater than the acute serum, what does that indicated.
Baron von Quacko, famous tag-team wrestler, has a famous "sleeper hold" that he uses on his opponents. Using only a single digit on each hand, he presses on his opponent's neck until his opponent passes out. What are the structures on which he is ..
Which parts of a eukaryotic gene are transcribed?
Histone proteins from many different eukaryotes are highly similar in their amino acid sequence, making them among the most highly conserved eukaryotic proteins. What functional properties of histone proteins might limit their diversity?
Hydrogen peroxide is toxic to cells, yet is a common byproduct of the reactions the occur inside the body. How can this compound by changed to become non-toxic?
The indole test is an example of a deamination reaction. Draw the specific biochemical reaction that is the basis of the indole test.
q. the panels hang perpendicularly from the side of a dock. animals such as crabs and snails live on the dock and can
Hurler syndrome is an autosomal recessive disease caused by a deficiency of the enzyme alpha-L-iduronidase. Affected individuals are mentally retarded.
If you generated a mutation that sped up rate at which the node moves toward the posterior, what effect would it have on somite formation?
Determine strains of E. coli, B-galactosidase will be Absent in both the presence and absence of lactose or Abundant only in the absence of lactose.
Assume you go back in time to Mendel's pea garden, and you come across an experiment where he has crossed purple with white flower colored pea plants.
Consider the meaning of the term "ecology." How do the ideas of energy and chemical cycles, community structure, biodiversity and succession fit together to form the basis of the way the natural world works?
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