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Lysozyme was assayed at bacterial cell wall, [S] = 2.5x10-8 g. The Km for the substrate = 6x10-3g. At the end of one minute 2.5% of the cell wall hydrolysed. Answer the following questions using this information:
a) What percent of bacetrial cell wall will be hydrolyzed at the end of 3 minutes assuming product formation is in 1st order of reaction?
b) What will be a concentration of hydrolyzed product at the end of 4 minutes?
c) Determine maximal velocity (Vmax) with the enzyme concentration used.
Explain a species using criteria that are used to determine if two populations are the same species along with a discussion of the procedure of speciation with instance.
It is determined that the human immune system synthesizes 10 8 – 10 10 antibodies. The portion of a typical active in binding antigen consists of two variable domains each containing one hundred amino acids.
Mitochondria are now day descendants of endosymbiotic bacteria. How many different RNA polymerases would you expect to find in Drosophila mitochondria?
Bones vary in mechanical properties. Where on the range of mechanical properties for bone do you think this bone would fall? Address at least elastic modulus, yield stress, and yield or ultimate strain.
All enterics are facultative anaerobes; that is, they have both respiratory and fermentative enzymes. What color results would you expect for organisms in O-F glucose media inoculated with an enteric.
In the cell bio lab, we use company manufactured gels, however you can make you own polyacrylamide gels. Discuss all of the ingredients found in an SDS-PAGE gel.
Einstein once predicted that a sugar molecule glucose had a length of 1 nm. If glucose has a hexagonal structure, draw a picture of the 24 atoms in glucose.
What is the difference between conservation of amino acids vs. the identity of amino acids residues in a multiple sequence alignment?
Assume you are provided an isolated muscle with its motor nerve intact. The preparation allows you to make electrophysiological analysis from the nerve and muscle as well as mechanical measurements of muscle contraction.
The gene has 32 exons. Removal of exons 3-5 should alter the reading frame and produce a protein with an early stop codon. How might removal of three exons lead to a stop codon that occurs in exon 6 instead of in exon 32?
Describe and discuss the difference between heme and nonheme iron. How can the efficiency of absorption be increased for both types of iron?
You have a 10x enzyme buffer that you are to use in a particular reaction. The total volume of the reaction is 30 micro liters. Determine how much of the 10x buffer should you add so that the final concentration of buffer in the reaction is 1x?
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