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You are working with a Gram positive bacterium that can be grown in Tryptic Soy Broth, a complex medium, or in a defined medium containing glucose, ammonium sulfate, potassium phosphate, ferric chloride, sodium phosphate and magnesium sulfate.
You suspect that the amount of iron in the defined medium would affect the growth rate. Describe an experiment you could do to test this hypothesis. Be sure to describe the growth conditions and the methods you would use to determine if iron affects the growth rate.
DIagram a chain of two RNA nucleotides. Use R for the nitrogenous bases. Then, briefly describe why RNA is usually single-stranded and why it is less stable than DNA.
Shifting the two bound tRNA from the A and P sites to the E and P sites of the ribosome involves:
Imagine that the sample of sediments are cemented together to form a sedimentary rock. After that, that rock is heated and compressed into a metamorphic rock.
Alkaptonuria is a metabolic disorder in which the affectd persons produce black urine. Alkaptonuria results from an allele.
In detail compare and contrast photosynthesis and cellular respiration. You should include the types of reactions that occur, the chemical equations of the two processes.
In the presence of high intracellular concentrations of tryptophan, only short transcripts of the trp operon are synthesized because of attenuation of transcription 5' to the structural genes.
A bowl of potato salad was left on picnic table for three hours on hot summer day. The microbial load was 300 CFU/g when the salad was put on table.
You look up in fall of the year and notice birds flying south. Describe the approaches scientists use to learn about nature. Design different measurements you could take and some experiments you might make in order to more fully describe this observa..
List and describe at least three cellular features of bacteria that could be targeted to inhibit or kill bacterial pathogen. Which is a significant flaw in this experimental set-up.
Describe how you would identify E. coli mutants that cannot utilize galactose as a carbon (energy) source. You are given a test tube containing E. coli grown under full nutrition, a spreader, filter paper, Petri plates with agar gel containing var..
Assume you are provided a solution of 0.1 M NaCl. What is the concentration of that solution in mg/ml? In %w/v?
Explain the phenomenon of drug resistance from the standpoint of microbial genetics inlcude a description of R plasmids/factors.
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