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Mutations in the rII region of a bacteriophage T4 prevent its growth on E. coli strain K. You obtain numerous mutations in this region and want to determine how many genes are located there. You perform double infections of E. coli K with the different pairwise combinations of mutations both in cis and in trans-configuration. (Recall that cis-configuration means that two mutations are located in the same phage genome, and trans means that they are located in different genomes).
Cis-test Trans-testrIIA rIIB / + + ==> growth rIIA + / + rIIB ==> growthrIIA rIIC / + + ==> growth rIIA + / + rIIC ==> growthrIIB rIIC / + + ==> growth rIIB + / + rIIC ==> no growthrIIA rIID / + + ==> no growth rIIA + / + rIID ==> no growth
What can be concluded from this experiment?
Discuss the differences between a gymnosperm's secondary growth and an angiosperm's secondary growth? how could you tell the stems apart?
Explain the structure and functions of keratin. Does every layer of the epidermic contain keratin? Why or why not? Please provide a justification for your answer.
Compare the recipes of nutrient agar and HE agar. If an organism can grow on both media, on which would you expect it to grow better? Why?
Are temperature gradients present in all biomes? What is one example of an adaptation to a colder temperature range?
A hurricane in Caribbean devastates a small isolated island, destroying all but about a dozen of once dominant turpentine trees on the island.
Which one of the following statements about archae is correct.
Does double-stranded RNA ever exist in a bacterial or a eukaryotic cell? What might this indicate and what might a cell do about this potentially bad situation?
One-fourth of their sons will be color blind and have blue eyes, 1/4 of their sons will be color blind and have brown eyes, 1/4 of their sons will have normal color vision and blue eyes, 1/4 of their sons will have normal color vision and brown ey..
In bio lab we studied corn on the cob and the four different traits which were purple, smooth , yellow , wrinkled corn. question is the traits i investigated in this excercise inherited though single gene, few genes, many genes?
It is a great day at the lab. You have identified an E. coli F- strain that cannot grow without biotin (bio-). Unfortunately, the next day a rivaling postdoc "accidently" mixed your strain with another strain ‘X' that cannot grow without arginine ..
A young researcher at National Institute Health and interested in eye development, and hoping to find a cure to blindness in your lifetime
Think about an artificial animal cell which contains 0.3 mmol/L NaCl (MW: 58.5 g/mol) and 0.01 mmol/L glucose (MW: 180 g/mol) in the cytoplasm.
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