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You are to convert the 5 mM fumaric acid in a waste stream into malate using fumarase immobilized on nonporous spherical particles. The enzyme has a turnover number of 23/sec, and a Km of 1.55 mM. The immobilized enzyme particles are 1 mm in diameter, and they contain 99.4 x 10-4 mg enzyme/cm2 of support. The mass transfer coefficient is 2 x 10-4 cm/sec. The molecular weight of fumarase is 48,000.a) What is the fumaric acid concentration on the surface of the particles?b) What is the effectiveness factor for the immobilized enzyme?c) What should the reaction rate be to treat 1 liters of the waste stream per day?d) What surface area is required to yield the required reaction rate?e) How many particles would be required?
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If the control is solid and an inoculated tube is liquid, is it acceptable to read the results before the complete incubation time has elapsed? Why or why not.
Determine which chemical groups of nucleotides react to form a covalent phosphodiester bond?
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MacConkey Agar gives two results: growth and color change. What does each of the two results tell you about the characteristics and/or biochemical abilities of the organism being tested?
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During organ transplantation, doctors are careful to ensure a close tissue match between the MHC types of donor and recipient.
Find the time td when the death occurs assuming this trigonometric environmental temperature and give the time on the clock. td = . Time on the clock = : AM, where the minutes are a decimal value with at least 4 significant figures.
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