Determine dissociation constant for the enzyme inhibitor

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Reference no: EM1394781

You run a set of reactions to determine the reaction rate as a function of substrate concentration and notice that the rate decreases as your substrate concentration increases. You suspect that either the substrate or the product is inhibiting the reaction. Describe the experiments you would conduct to determine which compound is inhibitory. If the product is inhibitory, how would you determine both the type of inhibition and the dissociation constant for the enzyme-inhibitor (or enzyme-substrate-inhibitor) complex, KI? [Note: You need to describe both the data you would collect as well as how you would use that data to solve this problem.]

Reference no: EM1394781

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