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You are provided with the following portion of a protocol:
1) Figure out your plan for making each solution required in the protocol. Be certain you FINISH with the required volumes of EACH solution
a. Calculate what is needed to make the 4 mg/ml solution
b. Calculate what is needed to make the 400 ug/ml working solution
c. Calculate what is needed to make the 40 ug/ml working solution
Reaction Rate as a Function of Substrate Concentration
Reaction mixture:
Assay 6 7 8 9 10 11
Lactate (mM)
9
3.15
1.8
1.35
0.9
0.45
1.5 M Tris pH 8.8
345 ml
735 ml
825 ml
855 ml
885 ml
915 ml
15 mM Lactate
600
210
120
90
60
30
38 mM NAD
250 mg/ml LDH
25
2) Looking at the "Reaction Rate as a Function of Substrate Concentration" section in the lab protocol, which assay would you predict to have the highest LDH activity and why?
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