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You have isolated five yeast mutants (a - e) unable to metabolize galactose. All of the mutants fail to transcribe the GAL2 gene, which encodes the enzyme for the first step in metabolizing galactose. You want to determine whether each mutant isa cis-acting mutation of a regulatory sequence for GAL2 itself;a trans-acting mutation in GAL4, which activates transcription of GAL2 and other enzymatic genes in the galactose metabolic pathway; ora trans-acting mutation in GAL3, which interacts with GAL80 to de-repress GAL4 when galactose is present. In analyzing each mutant, you make the following observations. NOTE: Yeast are normally haploid, but the diploid cells resulting from mating can persist as diploid colonies until induced to undergo meiosis.Mutant a: When this mutant is crossed to a known GAL4 recessive mutant strain, the resulting diploid yeast colonies are all unable to metabolize galactose.Mutant b: You do a cross and find this mutation maps to the vicinity of GAL3, and not to GAL4 or GAL2.Mutant c: A double mutant haploid with both this mutation and a GAL80 mutation restores galactose metabolism, and GAL2 is transcribed even when galactose is absent.Mutant d: All other enzyme-encoding genes (other than GAL2) in the galactose metabolism pathway are transcribed normally in this mutant.Mutant e: The DNA sequence region containing the UAS sites upstream of GAL2 is deleted in this mutant.Part AWhich mutant or mutants show evidence of a cis-regulatory mutation of GAL2?Select all answers that apply.
Select all answers that apply.mutant amutant emutant bmutant dmutant cSubmitMy AnswersGive UpPart BWhich mutant or mutants show evidence of a mutation in GAL4?Select all answers that apply.
Select all answers that apply.mutant cmutant amutant bmutant emutant dSubmitMy AnswersGive UpPart CWhich mutant or mutants show evidence of a mutation in GAL3?Select all answers that apply.
Select all answers that apply.mutant dmutant amutant bmutant cmutant e
Design an experiment to demonstrate that DNA replication is semi-conservative. List two markers that one can use to label the DNA.
With respect to the case-control studies compare the usefulness of the hospital and neighbourhood (community) controls. Your comparison must involve a comparison of the cost, feasibility, co-operation of the controls, recall bias, selection of contro..
While walking throughout a forest you notice that someone has carved his or her initials into the bark of a tree. The initials are exactly 1.5 meters from the ground. How far from grounds will the initials be next year and the year after that? Why? E..
The doctor writes an order for 125 mg of a medication. The label on the vial states "50 mg per ml". How many ml should the nurse administer?
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The phosphate system serves to buffer intracellular fluid of cells at physiological pH because pK2 (7.20) lies near the normal pH range of 6.9-7.4. If the total cellular concentration of phosphate is 19mM and the pH is 6.9, what is the concentrati..
The measurements to be comparable in both preparations. Illustrate one experiment you could perform to demonstrate such lack of equivalence of the two cells in the zygote. Noticeably state the expected outcome of your experiment in the reply you prov..
Describe why people confined to wheelchairs as of paralyzed lower limbs have thin, weak bones in their thighs and legs.
Women's bladders, on average, are smaller than those of men. The distribution of bladder volume in women is approximately N (400, 75) in milliliters. Find the proportion of women's bladders with a volume between 500 and 600 ml.
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Complete a chart by identifying six actions that reduce the risk of infection and strengthen the body's immune system. In the second column, explain how the action reduces risk of infection and strengthens the body's immune system.
Discuss how modern genetic technology may lead to personalized medicine. Give two or more specific examples of the benefits of personalized medicine.
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