Determine ability of pigment to absorb different wavelengths

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Reference no: EM131400875

Write one page organic chemistry report.

Follow the instractions on the attachmen.

The experiment consisted of using a spectrophotometer to determine the ability of a pigment to absorb different wavelengths of light in chlorophyll extract. An absorbance graph was to be made and the hypothesis was that the spectrum graph of chlorophyll would have a dip in the green area since the green pigment will be reflected. Using alcohol as the control and chlorophyll extract as the test subject, both materials were placed into a spectrophotometer and wavelength pigments were determined between 400 and 700. Results obtained were lower numbers in the green region and had higher numbers in the blue and red region. Therefore, that is proof that chlorophyll reflects the green wave lengths between 490 and 590. Also, the best absorption of light by chlorophyll is in the red, blue and violet waves.

Introduction

Photosynthesis is the way plants produce food. There are two stages of photosynthesis. First, the light cycle converts light energy to chemical energy. Secondly, the Calvin cycle uses the chemical energy from the light reactions to make sugar. Light travels in wavelengths that range from 380 nm to 760 nm. Pigments are substances that absorb visible light. Different pigments absorb different wavelengths of the light (See Table 1). Wavelengths that are absorbed disappear and wavelengths that are reflected are seen. Plants with chlorophyll appear green since the green wavelengths are reflected and not absorbed. Therefore, the greatest absorption should be in the red, blue, and violet wavelengths. Chlorophylls a and b can absorb a maximum of 600-675 and 400-475 nm ranges and will absorb light in two regions of the spectrum. To test the absorption of pigments a spectrophotometer is used. A graph called an absorption spectrum plots the pigment's light absorption versus the wavelength.

Laboratory Reports

Laboratory reports are an overview of the experiment written in a paragraph. They are similar in form to an abstract. The report should provide a brief introduction that includes the purpose of the experiment. The methods used in the experiment should include enough detail to understand what was done, but not as much detail as a methods section of a research paper. The results of the experiment should include the general outcome and include any error calculations. The discussion should give a brief summary of the experiment and include a brief discussion of the sources of error.

The report should be no longer than 1 page, single spaced, 12 point font (similar to an abstract).

Reports are due on the day of the next lab unless otherwise stated.

Attachment:- Chlorophyll_Rlja.xlsx

Reference no: EM131400875

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