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Detergents are critical reagents in cell biology

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1. Detergents are critical reagents in cell biology and biochemistry. Trion-X100 is a detergent. What is a detergent and find the chemical structure of Triton X-100? What features do you observe in this structure that might explain how it functions as a detergent?

2. The total number of cells and % Trypan-positive cells can both be indicators of cell damage. However, cell number can be a better indicative of extensive damage relative to staining with trypan. What do you think this means?

3. A lab that studies bacterial toxins that form pores in mammalian cells hired you as a lab technician. You are tasked to help manage and order reagent inventory. An order request was put forward to purchase cholera toxin from Vibrio cholerae, the bacteria that causes cholera. One of your favorite companies is a company called Sigma Aldrich (now MilliporeSigma).

a. Check their website to see if they sell this toxin.

b. What is the catalog number for this?

c. What size options are there to purchase and does the company list the price?

d. Find out the specifications for this product (is it powder/liquid, solvent, stability/re-test recommendation.

e. Download and include the safety data sheet (SDS) for this product. Indicate in your report, what kind of dangers there is to you if exposed to cholera toxin. What should you do if you inhaled this substance?

Reference no: EM133457360

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