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Recent advances in molecular biology have resulted in a new therapeutic process called RNA interference (RNAi). The purpose of RNAi is to silence the expression of unwanted genes by targeting the destruction of the mRNA transcript. Small interfering RNA (siRNA) are short pieces of double-stranded RNA engineered by a scientist. Inside the cell, the double-stranded siRNA unwinds, the anti-sense siRNA strand complimentarily binds to the mRNA of interest, which is then headed for mRNA degradation. As a result, protein is not translated and expressed. In treating colorectal cancer you are interested in silencing the expression of a mutated gene called APC. The template strand of the APC gene is 3'-ATTGCGTAG-5' . For your RNAi experiment, what is the seuqence of the anti-sense siRNA strand needed to silence APC expression?
It is a very curcial concept to understand how the immune response is mounted against viruses, bacteria, protozoans and helminthes. For an effective immune response, both innate and adaptive immunity should work together.
This Project report elaborates a critical review of important elements attached to Advanced Glycated End Products (AGEs). It is very crucial to understand the process called Millard reaction.
Soil stabilization is the permanent physical and chemical alteration of soils to enhance their physical properties. Stabilization can increase the shear strength of a soil and control the shrink-swell properties.
This assignment has three parts which contains questions related to Microbiology. It contains basic principles of microscopy, staining techniques in microbiology and microbial growth in the food industry.
Lipid metabolites are often seen as key elements in cellular signaling. Is this unique? Please provide several examples of the function of lipids as key elements in signal arrays and list the biologic functions these signals affect?
Please describe how one might search for chemical structure, biologic function relationships, involving small molecular weight lipophylic compounds. Provide one example.
Write a case study which detailing a scenario of a patient being investigated in the Haematology laboratory.
The use of PCR and genetic approaches in biotechnology
Glucose oxidase is an enzyme that can be used for measurements of glucose levels by combining this reaction with an oxygen probe.
What phenotypic ratio would you get if you crossed a white mouse and a heterozygous brown mouse?
Prepare an essay on nosocomial infection.
To increase the awareness of monitoring and recording the blood pressure of patients and practice measuring blood pressure in a safe environment.
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