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Here is the amino acid sequence of the enzyme (in single letter code):
MAAMDMDQFLAAAIDAAKKAGQIIRKGFYETKHVEHKGQVDLVTETDKGCEELVFNHLKQLFPNHKFIGEETTAAFGVTELTDEPTWIVDPLDGTTNFVHGFPFVCVSIGLTIGKVPVVGVVYNPIMEELFTGVQGKGAFLNGKRIKVSAQSELLTALLVTEAGTKRDKATLDDTTNRINSLLTKVRSLRMSGSCALDLCGVACGRVDIFYELGFGGPWDIAAGIVIVKEAGGLIFDPSGKDLDITSQRIAASNASLKELFAEAMMLTAA
Design two degenerate primer sequences that will amplify the entire Open reading frame (ORF) of the corresponding gene in a PCR reaction. To facilitate the cloning of the gene after PCR amplification, place a restriction endonuclease site at each end of your primers. You may choose any restriction endonuclease site you like, or use EcoRI (GAATTC). Hints: restriction endonuclease sites are cleaved more efficiently when 1-2 extra nucleotides are included at each end; these extra nucleotides are usually Gs or Cs. You need a forward and a reverse primer. In general, primers that are 18-30 nucleotides long are suitable for PCR amplification. Write out your primer sequences 5’ to 3’.
1. Forward primer sequence:
2. Reverse primer sequence:
Use the data provided below compute the resting membrane potential of this set of cells. Is the inside negative or positive relative to the outside ?
What are the causes of gastroesophageal reflux disease (GERD)? What is the typical prognosis for GERD and how is it treated.
Which of the following movements is an example of extension? bending forward at the waist, kneeling, raising your arm laterally.
The "Primordial soup" includes the mixture of elements and gases which are thought to have started life on the Earth. Based upon what you have learned related to the cells and complex molecules, do you feel this theory makes sense?
Which condition is best described while the ovaries shut down their production of eggs and hormones like estrogens and progesterone approximately 30 years after they started.
Substrate-level phosphorylation occurs within the metabolic pathway where sufficient energy is released by given chemical reaction to drive the synthesis of ATP from ADP and phosphate. In which the metabolic pathways are substrate-level phosphorylati..
The endosymbiosis theory discusses that mitochondria and chloroplasts were originally prokaryotic cells that were engulfed by ancestors of current day eukaryotic cells.
Joshua Ledergerg said, "Our only real competition for domination of planet remains the viruses." In what respect might you make a similar argument about insects.
In mid afternoon, they are on it's under surface, each caterpillar hanging by a few legs. Offer a hypothesis to explain this behaviour. How would you test it.
A 2-amino-purine can be incorporated into DNA in place of adenine. It can pair equally well with thymine or cytosine. What type of mutation do you expect it to cause.
define how a specific change in the mRNA sequence
What are the targets and mechanisms of action of antiviral drugs to influenza and why are there so few antivirals to influenza available?
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