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I have to write a lab report about ELISA (Direct and Sandwich). One of the tasks is to draw histograms from the data we obtained.Basically we have three serums and we are looking for IgG and IgE. Each sample has two wells so two data is available.After finding the average of G1, E1, G2, E2, G3 and E3 plotting thehistogram is not a problem. But the next question is asking us todraw error bars!!! I have gone through lots of articles andjournals and everyone uses error bars in their ELISA datapresentation but no one describes how you would do one?Any ideas about how to place error bars into histograms? Do I haveto find the Standard Deviation? Student's t Test? Percentage errorbar?
Another question: Explain the rationale of the design of direct binding and sandwich ELISA. And advantages of using a peptidemarker instead of a full length antigen in immunodiagnosis.
Dynein is missing from the cilia and flagella of individuals with a specific inherited disorder. These individuals have severe respiratory problems and, if males, are sterile. What is the structural connection between these two symptoms?
Clades are defined as groups that include an ancestral species and all of its descendants. Choose the following group type that is NOT considered a true clade?
If two F1's are crossed what would be the genotypic ratio of the F2 generation (FFSS: FFSs: FfSS: FfSs: FFss: Ffss: ffSS: ffSs: ffs)?
The integrity of the plasma membrane is essential for cellular survival. Could the immune system utilize this fact to destroy foreign cells that have invaded the body?What physical and metabolic constraints limit the cell size.
have you ever wondered why you get tired much faster on warmer days than on cold ones or why you become dizzy and weak
After Drosophila DNA has been treated with a restriction enzyme, the fragments are inserted into plasmids and selected as clones in E. coli. With the use of this "shotgun" technique, every DNA sequence of Drosophila in a library can be recovered.
Plasmids, which are associated with membrane proteins, depend on bacterial chromosomal DNA for replication. If the genome contains ~30,000 genes, about how many genes would be removed by a deletion of DNA that might be detected by karyotype analysis.
Which unit would you most likely use to measure the difference between the elevation of an airplane in flight and the height of a car on a road?
Concerns surrounding both dehydration and over hydration. Focus on water, sodium and potassium. How reliable is thirst as a guiding factor in fluid intake?
The pattern of coat coloration in dogs is determined by the alleles of a single gene, with S being dominant over s. Black coat color is determined by the dominant allele A of a second gene, and homozygous recessive aa animals are tan.
q1. a biologist discovers an alga that is marine multicellular and lives at a depth reached only by blue light. this
What are some decisions to be made when determining the types of carbohydrates to be selected for a sensible diet? What factors are used in making these decisions?
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