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I want to design a diagnostic test to distinguish a mutant allele from a wild type allele, as in the two allele depicted below. I have the choice of using two restriction enzymes (A and B) with target sites marked by the placement of A and B on th etwo alleles below. The resultant fragments friom the digestion of the two alleles are indicated below in parantheses. Suppose you design a PCR test for detecting one or the other allele using a single set of primers. For your test, which of the following represent all valid choices of restriction enzymes to detect a difference between teh two alleles?___(100)__A__(300)__A__(100)___ WT_______(500)____B____(500)_____ MutantA. only AB. only BC. neither A nor BD. A and BE, you can only use A and B together
Do different methods of classification all suggest the same phylogenetic history?
After the complete oxidation of glucose via glycolysis and the Krebs cycle, most of the energy once stored in a glucose molecule is now stored in molecules of ATP, NADH and FADH2. Remembering that each energy conversion is not 100% efficient, what..
Describe how hydrostatic pressure in the pseudocoelomic fluid affects functioning of the body–wall muscles in nematodes
Determine which of the following pairs of functional groups CANNOT react with each other by a dehydration reaction?
germination of fern spores does not require mRNA synthesis for protein synthesis. Propose a mechanism that would allow germination without mRNA synthesis in the spore.
What is responsible for the difference in potential energy between carbohydrates and carbon dioxide?
q. select one animal species. your goal is to explain the way in which an evolutionary change might occur for a
Certain chemicals block ATP synthesis by allowing protons and other ions to "leak across membranes," disrupting the charge and protein gradients established by electron flow through an electron transport chain.would this observation support the che..
What are the roles of ACC2 and biotin in the Krebs cycle-- specifically how do they interact with each other during the cycle?
What impact does the inability of DNA polymerase to synthesize DNA in the 3'-5' direction have on DNA replication? What solutions have evolved to overcome this limitation?
What do mean by RFLPs, VNTRs, and SNPs.
The branching gastro vascular cavity of cnidarian and Platyhelminthes: Do these animals have distinct boundary organs? Compare and contrast the basic body plan of these animals? How does the fluid "circulate" without one or more pumps? Explain why..
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