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You decide to design an assay for flu infection detection. HA protein is only present on the surface of influenza viral particles so you choose to design an ELISA assay to detect the presence of HA in human blood samples. You will use THREE different antibodies for the assay. The experimental procedure is as follows: Step 1. Incubate your assay plate with antibody (I) solution overnight to coat the surface of the assay plate with antibody (I). Remove antibody (I) solution and wash the plate extensively. Step 2. Add blood samples to the coated plate and incubate for 30 min. Remove blood sample and wash the plate extensively. Step 3. Add antibody (II) solution to the plate and incubate for 30 min. Remove antibody (II) solution and wash the plate extensively. Step 4. Add antibody (III) solution to the plate and incubate for 30 min. Remove antibody (III) solution and wash the plate extensively. Step 5. Add TMB substrate to the plate and monitor color development. In your lab, you have the following reagents on hand: Rabbit anti-HA polyclonal antibody Mouse anti-HA monoclonal antibody Rabbit anti-HA monoclonal antibody Goat anti-rabbit polyclonal antibody conjugated to HRP Goat anti-mouse polyclonal antibody conjugated to HRP You decide to use Rabbit anti-HA polyclonal antibody to coat the surface of your assay plate. What would be an appropriate negative control sample for your ELISA assay? (1pt) What is the expected colorimetric result from the negative control in your assay? (2pt) What would be an appropriate positive control sample for your ELISA assay? (1pt) What is the expected colorimetric result from the positive control? (2pt) Indicate whether or not the following antibody combinations could be used to successfully perform your assay. If a pair can't be used successfully, indicate why not. (6pts) ? Rabbit anti-HA polyclonal antibody as antibody (II) and Goat anti-rabbit polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no ? Mouse anti-HA monoclonal antibody as antibody (II) and Goat anti-rabbit polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no ? Mouse anti-HA monoclonal antibody as antibody (II) and Goat anti-mouse polyclonal antibody conjugated to HRP as antibody (III) Circle one: yes no
How would gene therapy be used to treat SCID [severe combinedimmunodeficiency syndrome]?I know that most SCID cases are due to amissing enzyme, but I could not figure out exactly how gene therapycould be used to treat it.
Why are mutation and recombination important in the process of natural selection and the evolution of organisms?
What could be the pros and cons of populating the area with animals such as lions, cheetahs, hyenas, elephants, camels, etc.? Would that help bring back some of the lost biodiversity?
Assume that the locus is tightly linked to the centromere and that the trisomic chromosome forms a bivalent and a univalent (monovalent) at random.
Explain the differences between classical andatypical antipsychotics.
the affordable care act was designed as insurance reform and not health care reform. a significant opportunity was
q1. recognize the strengths and weakness of callaway taylormade titleist ping and nike. which company has a competitive
Hippogriffs can have black fur or gray fur. However, there is a high rate of mutation from black alleles into gray alleles. In a population of 1000 hippogriffs, if there are 800 alleles for black fur and the forward mutation rate (ie., from black ..
you have 0.05mL of an undiluted culture at a concentration of 3.6 X 10^6 CFU/mL. You then add 4.95mL sterile diluent. What is the dilution, and what is the final concentration of cells?
I need a drawing of a typical spherical virus which in addition to the standard structures, also has an envelope. It should be labeled three dimensions with parts removed to allow the viewer to see details inside the various layers. Thanks Will rate ..
Tina administered 1 liter of sterile distilled water IV to a patient. Predict the direction you would expect Tina's infusion to have produced in these parameters, and describeyour predictions.
While studying the Michaelis - Menton kinetics of enzymes in the TCA cycle, the synthetic hydrocarbon malonate was shown to act as a competitive inhibitor of one of the enzymes.
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