Design a forward and reverse primer

Reference no: EM133546287

Question: Cloning Primer design is a skill essential to the success of a PCR reaction. Let's say you want to repeat the subcloning we did this semester. Using the sequence from your section, choice of restriction enzymes and vector,

(a) please design a forward and reverse primer to amplify your sequence keeping in mind that you must use the given vector.

(b) Explain how the sense primer was (or was not) modified to fit the reading frame of the amplicon into the reading frame of the
vector.

The gene coding sequence for your section can be downloaded fromthe NCBI website. A photo of the multiple cloning site for the differentvectors is available on the course's Brightspace site (Hypo Vectors inthe Lab 2 section). The first enzyme must be used for the sense primer whilethe second will be used to design the antisense primer. Use 20 bp of codingsequence for your two primers.

Reference no: EM133546287

Questions Cloud

What type of cellular atrophy of the reproductive organs : A decrease of estrogen after menopause results in what type of cellular atrophy of the reproductive organs

Tabby and make sure she stays awake until story time : Tabby and make sure she stays awake until story time is over. Each paragraph must contain at least five well constructed sentences

How committed and serious you are to achieving success : These two elements go hand in hand, as these represent how committed and serious you are to achieving success.

Chondral stimulation of medial femoral condyle : Chondral stimulation of medial femoral condyle with anterior cruciate ligament reconstruction using bone patellar tendon bone

Design a forward and reverse primer : Design a forward and reverse primer to amplify your sequence keeping in mind that you must use the given vector.

What are you special skills sets : Are you equipped with all the skills for you to be successful for the future? What are you special skills sets?

Create a charitable reconstruction of the argument : Isabel wants to be a doctor. Which of the following premises should be added to create a charitable reconstruction of the argument

identify major milestones for your new venture : In this last development of your business plan, you will identify major milestones for your new venture as well as projections of financial performance.

What was the rate of these spontaneous contractions : What was the rate of these spontaneous contractions in your preparation (number of contractions per minute)? Please show your calculations

User Account

All Pages