Reference no: EM133334303

Assignment:

Read the following description of a lab procedure performed by a microbiology student and answer the question that follows.

Jane goes through the process of making a streak plate from a broth culture. First, she disinfects the lab area and then gathers the necessary equipment. Next, she labels the sterile agar plate with the organism and her initials. Then, she flames the inoculating loop. Without setting the loop down, she carefully opens the cap to the broth, flames the opening, and removes a loopful of culture without touching the tube with the loop. She flames the tube again and replaces the cap from her hand. She then streaks back and forth without overlapping in one quadrant of the plate while only lifting the lid partially. Next, she turns the plate and streaks a second quadrant passing through the previous streak just once. She turns the plate again and streaks the third quadrant by passing through the second quadrant once. She repeats this for the fourth quadrant. She then flames the loop before replacing it in the equipment box. She disinfects the lab table after cleaning up her lab area according to proper procedure.

Did Jane correctly perform this procedure? Explain any errors made along the way including a description of how any errors Jane made will affect the outcome of the procedure.