Reference no: EM133443174

Questions

1. Which statement describes how we can see DNA bands on an agarose gel.

a. Loading dye binds in between base pairs of DNA and fluoresces under UV light

b. Sybersafe binds in between base pairs of DNA and fluoresces under UV light

c. DNA is naturally fluorescent

d. The GFP fluoresces on the gel

2. What is the relationship between the size of a DNA fragment and the distance it migrates in an agarose gel?

a. Longer fragments migrate further down the gel because they are heavier

b. Shorter molecules have a greater charge therefore migrate more quickly through the gel

c. Shorter fragments migrate further down the gel because they are smaller and can move through the pores more easily

d. All DNA, regardless of size, will migrate to the same place because it is attracted to the positive electrode

3. Choose the correct phrase that describes how electrophoresis separates DNA.

a. DNA is negatively charged therefore moves toward the positive end of the gel box

b. DNA is negatively charged therefore moves toward the negative end of the gel box.

c. DNA is positively charged therefore moves toward the positive end of the gel box

d. DNA is positively charged therefore moves toward the negative end of the gel box.

4. Which is the correct sequence of steps involved in the process of a mini-prep?

a. harvest cells-- lyse cells-- denature DNA--- renature DNA--- bind DNA to a filter cartridge---- clean DNA--- elute DNA

b. harvest cells---- denature DNA---lyse cells---- renature DNA---- clean DNA----bind DNA to a filter cartridge-- elute DNA

c. lyse cells---- denature DNA---harvest cells---- renature DNA---- clean DNA----bind DNA to a filter cartridge-- elute DNA

d. harvest cells---- denature DNA---lyse cells---- renature DNA--- bind DNA to a filter cartridge---- clean DNA--- elute DNA eluting DNA

e. harvest cells----clean DNA--- denature DNA---lyse cells---- renature DNA----bind DNA to a filter cartridge-- elute DNA