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Dissolve a compound with a molecular mass of 272.05 in a 10-mL volumetric flask. You remove a 1.00-mL aliquot, place it in a 25-mL volumetric flask and dilute it to the mark. The absorbance of this diluted solution at 325 nm was 0.498 in a 1.000-cm cuvet. The molar absorptivity for this compound at 325 nm is 5325 = 5629 M1 cm1.
(a) What is the concentration of the compound in the cuvet?
(b) What is the concentration of the compound in the 10-mL flask?
(c) How many milligrams of compound were used to make the 10-mL solution?
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Neat, aliphatic, primary amines show two absorptions between 3400 to 3330 cm-1 and 3330 to 3250 cm-1 due respectively to asymmetrical and symmetrical stretch. Stretch at 3300 cm-1. Why do tertiary amines not absorb in this region of the spectrum?
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