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Questions:
1.) Describe two differences in procedure in the BCA assay and the Coomassie Blue Assay.
2.) What sample reaction volume did you use in your BCA assay?
3.) Describe the color change in the BCA assay.
4.) Write out the order of ALL sample tubes (protein standard and unknown) that you used when taking absorbances on the spectrophotometer.
5.) The BCA working reagent was prepared by instructor by mixing ____ parts reagent A with ____ parts reagent B.
6.) What wavelength is used to read absorbances in the BCA assay?
One of the most important skills a nurse needs is clinical judgment. Reflect on your own ability to critically think and the reading for this module.
Show your work. State the phenotypes of the two parents of the original wild-type fly.
Your book lists four different types of diversity: species diversity, genetic diversity, ecological diversity, and functional diversity.
What are the very basics of animals? Along with the evolutionary adaptations that have allowed them to flourish.
In detail explain the structure and function of the heart and blood flow. Include the conduction system and blood vessels. Provide your response specific to your audience who are a group of lay people without a background in science.
If an atom donates an electron, and becomes positive, another atom will accept the electron, and is negative.
A method for separating polypeptides makes use of their differentail solubilities. The solubility of huge polypeptides in water depends upon the relative polarity of their R groups.
An enzyme is complementary to the transition state - not to the substrate. What happens to the energy of catalysis (DGcat) when an enzyme is truly complementary to its substrate?
what are some vestigial structures in other organisms ( insects, whaeles, snakes, fish and amphibians) that can be traced to earlier ancestor?
A biologist inserts a gene from a human liver cell into the chromosome of a bacterium. The bacterium then transcribes this gene into mRNA
What is a/the "normal flora" of the human colon? Give a few examples of the bacteria found there.
Hypertonic, or hypotonic? Would water move into or out of the red blood cells (RBC) to maintain equilibrium?
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