Describe two differences in procedure in the bca assay

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Reference no: EM133423682

Questions:

1.) Describe two differences in procedure in the BCA assay and the Coomassie Blue Assay.

2.) What sample reaction volume did you use in your BCA assay?

3.) Describe the color change in the BCA assay.

4.) Write out the order of ALL sample tubes (protein standard and unknown) that you used when taking absorbances on the spectrophotometer.

5.) The BCA working reagent was prepared by instructor by mixing ____ parts reagent A with ____ parts reagent B.

6.) What wavelength is used to read absorbances in the BCA assay?

Reference no: EM133423682

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