Describe three differences between plant and animal cells

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Reference no: EM133710122

Assignment: Microscope Lab Report

A. Purpose of the Microscope

I. Define:

Magnification
Resolution
Contrast

II. Describe how to safely transport a microscope from the storage area.

B. Compound Microscope

I. Define:

Eyepiece
Arm
Course adjustment knob
Fine adjustment knob
Revolving nosepiece
Objective lenses
Stage clips
Stage
Iris diaphragm lever
Condenser
Light source
Base

C. Viewing Microscope Slides

I. Name the two types of slides used with the compound light microscope.

Temporary wet mount
Permanent mount

II. Compare the terms parcentral and parfocal.

Parcentral
Parfocal

III. In Lab, study the specimen orientation and answer the following questions:

1. With the scanning lens in place, the prepared slide is moved to the right. In which direction does the image appear to move?

2. The slide is moved away from you. In which direction does the image move?

D. Depth of Field

I. What diaphragm adjustment should be made to more easily determine the order of the colored threads?

E. Microscopic Measurement

I. What is the length of a specimen which fills ¼ of the diameter of the fields of view using:

Scanning power (40X)? μm

Low power (100X)? μm

High power (400X)? μm

II. In Lab Section V, Exercise 6, discover how to measure the diameter of the field of view.

1. What is the diameter of the field of view under scanning power (40X) in micrometers (µm)?

2. What is the diameter of the field of view under low power (100X) using the following equation:

Diameter of field of view of 100X (µm) = (4400µm) x (40X) = µm
100X

F. Cells

I. Identify and describe the two major cell types.

Characteristics	Prokaryotic Cells	Eukaryotic Cells
Genetic material
Cytoplasm

II. What three structures do all cells have in common?

G. Prokaryotic Cells

I. In Lab Section VII, Exercise 8, observe bacteria cells. Answer the following questions:

1. Which magnification is used to view the bacteria?

2. Can you see cell organelles within the cytoplasm?

3. Measure the approximate size (μm) of the bacterial cells.
μm

II. Draw and label a typical bacterial cell, then provide functions for at least five of the labeled structures. Sign, date and prepare an image of your drawing and include it with this lab report.

H. Eukaryotic Cells

I. Explain how to make a wet mount of cheek cells. What are two ways to increase contrast?

Task

I. Explain how the microscope is used as an instrument of measurement.

II. Determine the total magnification of a microscope that has an eyepiece lens (10X) and an objective lens (10X).

III. Name three differences between prokaryotic and eukaryotic cells.

IV. You are observing an onion epidermal cell under the microscope, but the image is faint. What could you do to improve contrast?

V. Compare the relative size of a bacteria cell, human cheek cell, and Elodea cell.

VI. Describe three differences between plant and animal cells.

VII. What is the purpose of adding safranin to a wet mount?

VIII. Do all plant cells contain chloroplasts?

Reference no: EM133710122

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