Describe the subunit composition of the native protein

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Reference no: EM1387522

Question: During gel filtration chromatography under native cconditions, a protein of interest elutes with a molecular weight between 500 and 550 kDA. During SDS-PAGE without 2-mercaptoethanol, the same protein migrates as a single band of approximately 13o kDA. When SDS-PAGE is carried out in the presence of 2-mercaptoethanol, the protein migrates as two bands of approximately 95 and 35 kDa. Based upon these results, describe the subunit composition of the native protein and the basis for your conclusions.

Reference no: EM1387522

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