Describe the subunit composition of the native protein

Reference no: EM1387522

Question: During gel filtration chromatography under native cconditions, a protein of interest elutes with a molecular weight between 500 and 550 kDA. During SDS-PAGE without 2-mercaptoethanol, the same protein migrates as a single band of approximately 13o kDA. When SDS-PAGE is carried out in the presence of 2-mercaptoethanol, the protein migrates as two bands of approximately 95 and 35 kDa. Based upon these results, describe the subunit composition of the native protein and the basis for your conclusions.

Reference no: EM1387522

Questions Cloud

Degree of precedence relationship among the activities : keep the level of detail for these activities general, rather than specific. Be sure to indicate some degree of precedence relationship among the activities

What is the speed of the bucket : A bucket filled with dirt of mass 22 kg is suspended by a rope that hangs over a pulley of mass 31kg and radius 0.15 m. everything is at first at rest.

Starting in year one and continuing forever : What is the CW, when i=10% per year, of $1500 per year, starting in year one and continuing forever: and $10,000 in year five, repeating every four years thereafter

For what wavelength of light would first-order dark fringe : The first-order bright fringe is a distance of 4.84 from the center of the central bright fringe. For what wavelength of light would the first-order dark fringe be observed at this similar point on the screen.

Describe the subunit composition of the native protein : In gel filtration chromatography under native cconditions, a protein of interest elutes with a molecular weight between five hundred and 550 kDA.

How to interconnect two areas using tenbaset : Given that both engineering and front office areas use 10BaseT (twisted-pair Ethernet), how might you interconnect those two areas? Explain what meduim guarantees immunity from interference?

Proportion of the time would he buy bananas : Mark makes it a policy not to buy bananas unless the price is 33 cents or less per pound. What proportion of the time would he buy bananas?

Overcoming generational poverty : Identify the complex factors involved in overcoming generational poverty. Explain challenges the aged face when there is a lack of access to employment.

Where is the image of tall noses located : You hold a spherical salad bowl 90 in front of your face with the bottom of the bowl facing you. The salad bowl is made of polished metal with the 30 radius of curvature. Where is the image of your 2.0 tall noses located.

User Account

All Pages