Describe the structure of dna

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Assignment:

Answer these question correctly and make sure your answer is easy to follow. I will give you a good review and excellent feedback. Do not copy and paste.

DNA dynamics (Nature of DNA)

Describe the structure of DNA
Identify the 4 nucleotides and how they are classified
Define hydrogen and phosphodiester bonds
Define melting temperature of a double stranded DNA molecule and understand how Tm influences processes like PCR
Understand how base composition of DNA influences melting temperature
Understand how energy in the form of heat and pH effect whether a DNA molecule is double or single stranded

Biobits (Central Dogma)/transcription and translation

Define the central Dogma
Define the term gene expression
Explain the function of transcription and translation in the process of gene expression
Explain what an exon, intron and promoter are and how they relate to gene expression
Be able to identify the start of transcription and translation of a gene
Compare and contrast a promoter with a start codon

What is a gene/Genome Browser lab

1. Define the term gene expression

2. Explain the function of transcription and translation in the process of gene expression

3. Explain what an exon, intron and promoter are and how they relate to gene expression

4. Compare and contrast a promoter with a start codon

5. Be able to explain what a genome browser is and the kinds of information that you can obtain from a genome browser.

Identify the number of genes in a particular section of DNA on a browser
Identify the number of exons and introns in a gene that is displayed on a genome browser
Identify an ORF (and describe what that is and why it is important in terms of gene expression)
Identify the reading frame of an exon?

Restriction enzymes

1. Describe the function of restriction enzymes

2. Be able to determine how many and the size of each fragment that would result from a restriction digest if given the size of the piece of DNA and the number of cut sites within that piece of DNA.

Associate certain fragment sizes with certain genotypes (like we did in the PTC lab).

Reference no: EM133478864

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