Reference no: EM132439722

Question 1: Describe the structure of a biological membrane, and name three functions of a membrane.

Question 2: What type of transport is responsible for allowing glucose across the membrane? Does this process require ATP?

Question 3: Cataracts are associated with a deficiency in which vitamin? Is this a water-soluble or fat-soluble vitamin?

Question 4: What is feedback inhibition? What type of enzymatic control is it? Give an example of feedback inhibition of an enzyme.

Question 5: An experiment was performed to determine the effects of an inhibitor on the breakdown of glycogen by an enzyme. In an accompanying experiment, the inhibitor was added to the glycogen-enzyme suspension and reacted using the same experimental conditions. The data obtained from these experiments is tabulated below.

Glycogen (mM)

Product Formed Glycogen only (mM/min)

Product Formed Glycogen and Inhibitor (mM/min)

0.5

22.6

15.9

1.0

32.1

24.9

1.5

38.1

28.2

2.0

41.2

35.1

2.5

44.7

40.0

3.0

48.5

43.3

a. Draw the Michaelis-Menten and Lineweaver-Burke plots of these data.

b. Determine the form of inhibition observed from these results, and explain your rationale for this form.

c. Determine the values for K_m and V_max from these results.

Question 6: What are Okazaki fragments?

Question 7: In the absence of lactose, where does the lac repressor protein bind in the lac operon? What happens when lactose is present?

To answer the following questions, read the paper by Talhaoui et al., as listed above, which can be found on the course home page.

Question 8: What hypothesis do the authors propose?

Question 9: Describe the DNA damage that occurs in human cells as a result of oxidative damage.

Question 10: How did the authors determine that base excision repair corrected errors from oxidative damage?

Question 11: What did the authors conclude about BER and NER repair systems?