Reference no: EM133231554

Assignment:

Part 1

Gram Stain and Exercise 8 Endospore stain and special stains Exercise 7 Gram Stain-Review the PowerPoint in iCollege, the appropriate part of the lab manual and watch this video of Gram Staining technique to answer Questions.

Describe the 4 steps of the Gram Stain procedure in order, starting after preparing a proper smear. Explain what happens at each step and what you would expect to see if you stopped the staining procedure at that step and looked at the slide after that step.

1. Which Gram Stain step is considered to be most critical (source of error)?

2. Describe what Gram Staining tells you about cells that is different from Simple Staining.

Part 2

Endospore Stain and Special stains

1. What primary stain (dye) is used in the Schaeffer-Fulton Endospore Stain?

2. What is the other part of this staining procedure?

3. What cell wall feature is the basis for the ZiehI-Neelson Acid Fast Stain? What is one disease that might be diagnosed using this stain?

4. Why is the bacterial smear not heat fixed in the Capsule Stain?

5. List Describe the 4 types of flagellar arrangement in bacteria.