Describe the preparation of distilled water

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Given a solution of .1 M HEPES in its fully protonated form, and ready access to .1M HCl, .1M NaOH, and distilled water. Describe the preparation of 1L of .025 M HEPES buffer solution, pH 7.8? The pka of the sulfonic acid of HEPES is 3 and the pKa of peperazine -NH+ is 7.55 at 20 degrees celcius

Reference no: EM13151480

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