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You are a geneticist who has a single copy of a gene that you wish to sequence. However, DNA sequencing requires multiple copies of the gene. Describe the steps you would take to solve this problem
Many organisms possessing many different lipases produce positive results on Tributyrin Agar. Is the inability of this medium to distinguish between these different enzymes a weakness in its specificity or its sensitivity?
How is the wavelength of light used to illuminate a specimen under a microscrope related to the power of resolution for a microscope?
Cells have several mechanisms to prevent the production and accumulation of truncated protein fragments. To illustrate why these fragments can be deleterious.
Growth factors and their corresponding receptors are commonly overexpressed and/or dysregulated in many cancers. First discuss the interplay between growth factors, their receptors, signaling cascades and the relationship of these three to cancer.
Many groups are using modified RNA with 2'-deoxy-2'-fluoro substitutions for riboses (i.e. a 2'-fluorine instead of a 2'-OH) to prevent RNA cleavage by ribonucleases.
Use human communication methods as an analogy for how cells communicate with one another. How are some of the ways cells communicate similar to the ways we communicate with other people or with an organization? How are they different?
Assume you were given fifty ml of sewage and asked to determine the number of bacteria in the sample. You began by taking five ml and putting it into 45 ml of sterile water.
Design an experiment in which you will test the effect of an acidic fluid on enzymatic activity. (Recall: enzymes are proteins.) To complete this project, it may be useful for you to review.
Most of us can't even imagine a world without oxygen. Though, as you learned earlier, chemically oxygen is a powerful oxidizing compound. What effect(s) would the increase in oxygen levels of atmosphere have on the organisms that existed at that time..
Imagine a gene that codes for a protein of 1000 amino acids.Compare the effect of a triple frameshift minus mutation thatoccurs at +19, +22 and +24 bp and one that occurs at +30, +1500,and +2900 bp.
Epigenetics refers to the modification of genes that affect the DNA sequence itself.
Consider a culture into which two E. coli strains are inoculated. Strain A is normal with g=21 min at 37 degrees C, and 42 mins at 27 degrees C. Strain B has a temperature-sensitive mutation, such that g=55 mins at 37 degrees C, and 39 mins at 27 ..
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