Describe the assay design, endpoint read

Assignment Help Biology

Reference no: EM133505590

Question: As a research investigator you are interested in studying the functional properties of the P2X7 receptor. So the first step you plan is to overexpress P2X7 receptor in HEK293 cells. Following this step, you are now requested to answer the following questions:

Design an assay to Quantitate the number of cells that are overexpressing the P2X7 receptor. Based on the assay that you choose to design, describe the assay design, endpoint read, types of controls you will need to include and the expected outcome/results of your assay to determine what % of your cells are overexpressing P2X7

Assay Design, instrumentation and readout
Controls
Expected Result/Quantitation

Reference no: EM133505590

Questions Cloud

Develop a system that will help addressing the issues : PRT547 Information Systems Practice, Charles Darwin University - develop a system that will help addressing the issues or opportunityies for the organization

What you have learned about these orders list two principle : List these five orders. Using what you have learned about these orders list two principle identifying characteristics for each order, and name two commonly

Most positive influence in your life : Discuss the organizational leader who has had the most positive influence in your life and explain why.

How cane toads might have directly or indirectly affected : Explain why you did or did not observe a change in the death rate of a crimson finches in their nests after the arrival of cane toads. Be sure to reference

Describe the assay design, endpoint read : describe the assay design, endpoint read, types of controls you will need to include and the expected outcome/results of your assay to determine

Design and implement security solutions using networking : LD7007 Network Security, Northumbria University - Critically evaluate threats to data and network security; design and implement security solutions

Discuss the pooled relative risk estimate was found : estimated that about one-third of prostate cancer cases can be present in men in their fourth or fifth decade of life without any clinical symptoms or signs.

Describe the tools and resource for teaching elementary math : Describe the following tools and resources for teaching elementary mathematics, including examples of use during instruction.

How it has shaped human evolution : Explain biocultural evolution and how it has shaped human evolution. Make sure to justify your answer with evidence.

User Account

All Pages